For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Informationen zur Lagerung
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Muzio, Polentarutti, Facchetti, Peri, Doni, Sironi, Transidico, Salmona, Introna, Mantovani: "Characterization of type II intracellular IL-1 receptor antagonist (IL-1ra3): a depot IL-1ra." in: European journal of immunology, Vol. 29, Issue 3, pp. 781-8, (1999) (PubMed).
Muzio, Polentarutti, Sironi, Poli, De Gioia, Introna, Mantovani, Colotta: "Cloning and characterization of a new isoform of the interleukin 1 receptor antagonist." in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 623-8, (1995) (PubMed).
The antibody reacts specificly with Human IL-1ra3. IL-1ra3 belongs to the IL-1 system which includes two agonists (IL-1alpha and IL-1beta), converting enzymes, antagonists, two receptors (IL-1 R I and IL-1 R II) and the IL-1 receptor accessory protein. Three molecular isoforms of the IL-1 receptor antagonist (IL-1ra) have been identified and cloned. Secreted IL-1ra (sIL-1ra or IL-1ra1) contains a classical leader peptide giving a released mature protein. Two intracellular isoforms, icIL-1ra type I (IL-1ra2) and icIL-1 ra type II (IL-1ra3), have no leader sequence, thus predicting that these proteins remain intracellular. IL-1ra3 may represent a reservoir of IL-1 inhibitors, released upon cell death, whose function is to limit the pro- inflammatory action of cell debris. Studies have shown that IL-1ra3 is expressed by various cell types upon exposure to inflammatory signals but most prominently by mononuclear phagocytes and keratinocytes.