Integrin beta 2 Antikörper

Produktnummer ABIN2191828

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch Integrin beta 2 in FACS, WB, Func und IA. Er zeigt eine Reaktivität gegenüber Maus und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für Integrin beta 2 Antikörper (ABIN2191828)

Target: Integrin beta 2 (ITGB2)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), Immunoassay (IA)

Klon: 5G11

Produktdetails anti-Integrin beta 2 Antikörper

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Applikationshinweise: For flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For agonistic studies in vitro, dilutions have to be made according to the amounts of ligand to be activated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-Integrin beta 2 Antikörper (ABIN2191828)

Banks, Rickert, Benedict, Ma, Ko, Meier, Ha, Schneider, Granger, Turovskaya, Elewaut, Otero, French, Henry, Hamilton, Scheu, Pfeffer, Ware: "A lymphotoxin-IFN-beta axis essential for lymphocyte survival revealed during cytomegalovirus infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7217-25, (2005) (PubMed).

Hehlgans, Müller, Stopfer, Männel: "Activation of the lymphotoxin-beta receptor induces NFkappaB-dependent interleukin-6 and MIP-2 secretion in mouse fibrosarcoma cells." in: European cytokine network, Vol. 14, Issue 2, pp. 103-7, (2003) (PubMed).

Lucas, Tacchini-Cottier, Guler, Vesin, Jemelin, Olleros, Marchal, Browning, Vassalli, Garcia: "A role for lymphotoxin beta receptor in host defense against Mycobacterium bovis BCG infection." in: European journal of immunology, Vol. 29, Issue 12, pp. 4002-10, (2000) (PubMed).

Details zu Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Andere Bezeichnung: Lt beta-Receptor

Hintergrund: The monoclonal antibody 5G11 reacts with the mouse lymphotoxin beta-receptor (LTbetaR), a member of the tumor necrosis factor (TNF) family. This receptor can be activated by its functional ligands lymphotoxin-alpha1-beta2 (LT-alpha1beta2) and LIGHT and is critically involved in controlling bacterial infections. In Mycobacterium infection, its role is related to bactericidal granuloma formation in the spleen, macrophage differentiation and the maintenance of the appropriate Th1/Th2 cytokine balance. In CMV infection, LTbetaR signaling induces the expression of IFN-beta in infected fibroblasts, resulting in viral stasis. LTbetaR plays an important role in lymphoid organogenesis and tumor development. The receptor is expressed on fibroblasts and stromal cells and at low levels in some myeloid cell lines. In the adult animal, the LTbetaR seems to be necessary for maintaining the splenic architecture and aspects of Ig formation and B cell follicular structure. When the LT-alpha1beta2/LTbetaR pathway is disrupted by genetic deletion, mice completely lack Peyer's patches. Also, LTbetaR induces cell death in some adenocarcinoma tumor lines in the presence of IFNgamma. The monoclonal antibody 5G11 directed against the mouse LTbetaR is able to activate the LTbetaR in an agonistic way and induces NFkappaB activation and secretion of MIP-2 and IL-6 in mouse fibroblast cells. Therefore, the monoclonal antibody 5G11 can be used to gain more insight into the expression pattern of the LTbetaR and also to investigate molecular mechanisms induced by LTbetaR activation.

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation