APEX1 Antikörper

Produktnummer ABIN219055

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch APEX1 in WB, IHC, ICC, IHC (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für APEX1 Antikörper (ABIN219055)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APEX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-APEX1 Antikörper

Spezifität: Recognizes human APE. Detects a band of ~37kD by Western Blot. Species cross-reactivity: mouse and rat.

Aufreinigung: Immunoaffinity purified

Immunogen: Recombinant human APE.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC (1:100 - 1:200), IHC, IHC-Fr, IHC-P, WB (1:300 - 1:1000)

Usage: Suitable for use in Immunocytochemistry, Western Blot and Immunohistochemistry. Also suitable for use in Apoptosis assay (following TUNEL assay using Boehringer-Mannheim TUNEL assay kit). Western Blot: 1:300-1:1000 on HL60 and HeLa cells using ECL. Antibody may be used 1-4X in the binding solution depending on the cells/cell lines. Immunohistochemistry: 1:100-1:200 on frozen and paraffin sections from human and rat tissues. Immunocytochemistry: 1:100-1:200 on cytospin preparations of cells fixed in paraformaldehyde. Apoptosis (TUNEL) assay: perform antibody staining AFTER the. TUNEL assay.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS. Contains no preservatives.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Andere Bezeichnung: APEX1 / APE1

Hintergrund: Name/Gene ID: APEX1

Synonyms: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APE1, APEX nuclease, Redox factor-1, AP endonuclease 1, APEX, APX, Protein REF-1, REF-1, REF1

Gen-ID: 328

UniProt: P27695

Pathways: DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus