APEX1 Antikörper

Produktnummer ABIN219055

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch APEX1 in WB, IHC, IHC (p), ICC und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für APEX1 Antikörper (ABIN219055)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APEX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-APEX1 Antikörper

Spezifität: Recognizes human APE. Detects a band of ~37kD by Western Blot. Species cross-reactivity: mouse and rat.

Aufreinigung: Immunoaffinity purified

Immunogen: Recombinant human APE.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC (1:100 - 1:200), IHC, IHC-Fr, IHC-P, WB (1:300 - 1:1000)

Usage: Suitable for use in Immunocytochemistry, Western Blot and Immunohistochemistry. Also suitable for use in Apoptosis assay (following TUNEL assay using Boehringer-Mannheim TUNEL assay kit). Western Blot: 1:300-1:1000 on HL60 and HeLa cells using ECL. Antibody may be used 1-4X in the binding solution depending on the cells/cell lines. Immunohistochemistry: 1:100-1:200 on frozen and paraffin sections from human and rat tissues. Immunocytochemistry: 1:100-1:200 on cytospin preparations of cells fixed in paraformaldehyde. Apoptosis (TUNEL) assay: perform antibody staining AFTER the. TUNEL assay.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS. Contains no preservatives.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Andere Bezeichnung: APEX1 / APE1

Hintergrund: Name/Gene ID: APEX1

Synonyms: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APE1, APEX nuclease, Redox factor-1, AP endonuclease 1, APEX, APX, Protein REF-1, REF-1, REF1

Gen-ID: 328

UniProt: P27695

Pathways: DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus