IL-5 Antikörper

Produktnummer ABIN218123

Der Ratte Monoklonal Anti-IL-5-Antikörper wurde für WB, ELISA, FACS, Neut und IF validiert. Er ist geeignet, IL-5 in Proben von Human und Maus zu detektieren.

Kurzübersicht für IL-5 Antikörper (ABIN218123)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL-5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut), Immunofluorescence (IF)

Produktdetails anti-IL-5 Antikörper

Spezifität: Recognizes mouse and human Interleukin-5 (IL-5).

Aufreinigung: Purified

Immunogen: Semi-purified mouse T cell clone supernatant.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, IF, Neut, WB

Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing mouse IL-5 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS. No preservative added.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: IL5

Hintergrund: Name/Gene ID: IL5
Family: Hormone

Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF

Gen-ID: 3567

UniProt: P05113

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour