IL-5 Antikörper

Produktnummer ABIN218122

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch IL-5 in WB, ELISA, FACS und IF. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für IL-5 Antikörper (ABIN218122)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL-5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-IL-5 Antikörper

Spezifität: Recognizes mouse and human Interleukin-5 (IL-5).

Aufreinigung: Purified

Immunogen: Semi-purified mouse T cell clone supernatant.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, IF, WB

Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 100 mM borate buffered saline,  pH 8.0. No preservatives or amine-containing buffer salts added.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: IL5

Hintergrund: Name/Gene ID: IL5
Family: Hormone

Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF

Gen-ID: 3567

UniProt: P05113

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour