P4HB Antikörper (AA 21-150) (Biotin)

Produktnummer ABIN2178365

Der Kaninchen-Polyklonal Anti-P4HB-Antikörper wurde für WB, ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet zum Nachweis von P4HB in Proben aus Human und Maus. }

Kurzübersicht für P4HB Antikörper (AA 21-150) (Biotin) (ABIN2178365)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser P4HB Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-P4HB Antikörper (Biotin)

Bindungsspezifität: AA 21-150

Kreuzreaktivität: Human, Maus

Homologie: Rat,Cow,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PDI

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Andere Bezeichnung: PDI

Hintergrund:

Synonyms: Protein Disulfide lsomerase, Cellular thyroid hormone-binding protein, PDI, Prolyl 4-hydroxylase subunit beta, p55, P4HB, ERBA2L, P4HB, ERBA2L, PDIA1, PO4DB, PDIA1_HUMAN.

Background: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9, the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).

Gen-ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism