HSP27 Antikörper (Biotin)

Produktnummer ABIN215210

Der Maus Monoklonal Anti-HSP27-Antikörper wurde für WB, IHC, ELISA, IP und ICC validiert. Er ist geeignet, HSP27 in Proben von Human und Affe zu detektieren.

Kurzübersicht für HSP27 Antikörper (Biotin) (ABIN215210)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP27 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-HSP27 Antikörper (Biotin)

Spezifität: Recognizes a ~27kD protein, corresponding to the apparent molecular mass of HSP27 on SDS-PAGE immunoblots. Species cross-reactivity: Human, monkey and fish (rainbow trout). Does not exhibit reactivity with rodent HSP25, alphaA, alphaB or beta-crystallin proteins.

Aufreinigung: Protein G purified

Immunogen: Purified HSP24 isolated from MCF-7, a human breast tumor cell line. Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, HSP27.

Type of Immunogen: Purified protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC (10 μg/mL), IHC (5 μg/mL), IP, WB (1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunoelectron Microscopy, Apoptosis Induction, and EIA/ELISA. Western Blot (ECL): 1 μg/mL. Immunohistochemistry: 5 μg/mL. immunocytochemistry: 10 μg/mL. Immunoelectron Microscopy: 1:50. Positive control: H1838-01: HeLa Heat Shocked Cell Lysate. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: HSPB1 / HSP27

Hintergrund: Name/Gene ID: HSPB1

Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27  kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067

Gen-ID: 3315

UniProt: P04792

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling