HSP27 Antikörper

Produktnummer ABIN215209

Dieses Maus Monoklonal-Antikörper erkennt spezifisch HSP27 in WB, IHC, ELISA, IF, IP und ICC. Er zeigt eine Reaktivität gegenüber Human und Affe.

Kurzübersicht für HSP27 Antikörper (ABIN215209)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-HSP27 Antikörper

Spezifität: Recognizes an ~27kD protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots. Species cross-reactivity: Human, monkey and fish (rainbow trout). It does not exhibit reactivity with rodent Hsp25, alphaA, alphaB or beta-crystallin proteins.

Aufreinigung: Protein G purified

Immunogen: Purified Hsp24 isolated from MCF-7, a human breast tumor cell line (7). Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, Hsp27.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC (10 μg/mL), IF, IHC (5 μg/mL), IP (12.5 μg/mL), WB

Usage: Suitable for use in ELISA, Western Blot and Immunofluorescence. Western: Blot (Colorimetric): 1 μg/mL. Immunoprecipitation: 12.5 μg/mL. Immunohistochemistry: 5 μg/mL. Immunocytochemistry: 10 μg/mL. Immunoelectron Microscopy: 1:50. Positive control: HeLa Heat Shocked Cell Lysate and Recombinant Human Hsp27 Protein.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, 50 % glycerol.

Konservierungsmittel: PMSF

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: HSPB1 / HSP27

Hintergrund: Name/Gene ID: HSPB1

Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27  kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067

Gen-ID: 3315

UniProt: P04792

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling