CD3 Antikörper (PE-Cy5)

Produktnummer ABIN2144340

Dieses Anti-CD3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD3 in FACS, IHC, Func, IF, WB und ICC. Geeignet für Human.

Kurzübersicht für CD3 Antikörper (PE-Cy5) (ABIN2144340)

Target: CD3

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist konjugiert mit PE-Cy5

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC)

Klon: 4AOKT3

Produktdetails anti-CD3 Antikörper (PE-Cy5)

Produktmerkmale: 4AOKT3 reacts with CD3, a 20-26 kDa molecule, which is expressed on all mature T lymphocytes (approximately 60-80 % of normal human peripheral blood lymphocytes), NK-T cells and some thymocytes.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]
/ul>

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3

Hintergrund: CD3 associated with the T-cell receptor a/b or g/d dimer also plays a role in T-cell activation and signal transduction during antigen recognition.* CD3 mAb is used to count mature T cells in blood.

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway