SIRT1 Antikörper (AA 207-256)

Produktnummer ABIN214144

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SIRT1 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Kaninchen, Affe, Rind (Kuh), Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Fledermaus und Schwein.

Kurzübersicht für SIRT1 Antikörper (AA 207-256) (ABIN214144)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Affe, Rind (Kuh), Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Fledermaus, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIRT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SIRT1 Antikörper

Bindungsspezifität: AA 207-256

Marke: IHC-plus™

Spezifität: Human SIRT1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Xenopus (92%) Chicken (85%) Zebrafish (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa207-256 of human SIRT1 (Q96EB6, NP_036370). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Turkey, Xenopus (92%), Chicken (85%), Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Andere Bezeichnung: SIRT1 / Sirtuin 1

Hintergrund: Name/Gene ID: SIRT1
Family: ADP-Ribosyltransferase

Synonyms: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1

Gen-ID: 23411

NCBI Accession: NP_036370

UniProt: Q96EB6

Pathways: MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling