PKC zeta Antikörper (N-Term)

Produktnummer ABIN213577

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PKC zeta in ELISA, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PKC zeta Antikörper (N-Term) (ABIN213577)

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC zeta Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PKC zeta Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Human PRKCZ / PKC-Zeta. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Monkey (95%) Marmoset, Mouse, Rat, Rabbit (89%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from near N-terminus of human PRKCZ / PKC-Zeta. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Monkey (95%), Marmoset, Mouse, Rat, Rabbit (89%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (2.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-3 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PKC zeta

Target: PKC zeta (PRKCZ) (Protein Kinase C, zeta (PRKCZ))

Andere Bezeichnung: PRKCZ / PKC-zeta

Hintergrund: Name/Gene ID: PRKCZ
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCZ, PKC-ZETA, PKC2, Pkcz, PKCzeta, Protein kinase c zeta, Protein kinase C zeta type, NPKC-zeta, PKC Zeta, Protein kinase C, zeta, Protein kinase Czeta

Gen-ID: 5590

Pathways: NF-kappaB Signalweg, RTK Signalweg, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling