CREB1 Antikörper (AA 5-24)

Produktnummer ABIN211132

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CREB1 in WB und IP. Er zeigt eine Reaktivität gegenüber Human, Schwein, Hund, Pferd, Kaninchen, Affe und Fledermaus.

Kurzübersicht für CREB1 Antikörper (AA 5-24) (ABIN211132)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reaktivität: Human, Schwein, Hund, Pferd, Kaninchen, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CREB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-CREB1 Antikörper

Bindungsspezifität: AA 5-24

Spezifität: Recognizes human CREB at 43kD. Species cross-reactivity: Broad species cross-reactivity expected due to conservation of immunogenic sequence.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%) Mouse, Hamster, Bovine, Opossum, Turkey, Chicken (95%) Rat, Xenopus (90%).

Aufreinigung: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide (C-SGAENQQSGDAAVTEAENQQ) corresponding to amino acids 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%), Mouse, Hamster, Bovine, Opossum, Turkey, Chicken (95%), Rat, Xenopus (90%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 mg/mL detects CREB from a HeLa nuclear lysate. Representative blot from a previous lot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CREB ( 0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear extract. Add 2.5 μL of 2- mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Immunoprecipitation: 5 mg (will immunoprecipitated CREB from 500 mg of HeLa nuclear extract).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.1 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Andere Bezeichnung: CREB1 / CREB

Hintergrund: Name/Gene ID: CREB1
Family: Transcription factor

Synonyms: CREB1, CREB-1, Transactivator protein, CREB

Gen-ID: 1385

UniProt: P16220

Pathways: TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation