CTNNB1 Antikörper (AA 29-49)

Produktnummer ABIN211079

Produktdetails anti-CTNNB1 Antikörper

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Bindungsspezifität: AA 29-49

Reaktivität: Human, Maus, Ratte, Hund, Huhn, Affe, Rind (Kuh), Zebrafisch (Danio rerio), Xenopus laevis, Pferd, Schwein, Hamster, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Spezifität: Recognizes b-Catenin, Mr 92kD. Species cross-reactivity: Human, bovine, rat, rabbit and mouse.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%) Stickleback (90%).

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide corresponding to the consensus GSK3 phosphorylation site (aa29-49, CGG-SYLDSGIHSGATTTAPSLSGK) of human b-Catenin (KLH coupled). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%), Stickleback (90%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC (1 - 5 μg/mL), IP, WB (0.1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 0.1-2 μg/mL. Detects b-catenin in an A431 total cell lysate. A431 total cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-b-Catenin (0.1 μg/mL). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg. Immunoprecipitates b-Catenin from 500 μg of A431 membrane fraction lysate. Immunocytochemistry: 1-5 μg/mL. Demonstrates positive immunostaining for b-catenin at cell-cell junctions of A431 cells fixed with 4 % paraformaldehyde and permeabilized with 0.25 % Triton X-100. Positive control: A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: CTNNB1 / Beta Catenin (CTNNB1 Produkte)

Synonyme: Jup antikoerper, LOC100217813 antikoerper, CTNNB1 antikoerper, CTNNB antikoerper, Bfc antikoerper, Catnb antikoerper, Mesc antikoerper, ctnnb antikoerper, id:ibd2058 antikoerper, wu:fb73e10 antikoerper, wu:fi81c06 antikoerper, wu:fk25h01 antikoerper, ctnnb1 antikoerper, CHBCAT antikoerper, MRD19 antikoerper, armadillo antikoerper, beta-catenin antikoerper, catenin beta 1 antikoerper, catenin (cadherin associated protein), beta 1 antikoerper, catenin (cadherin-associated protein), beta 1 antikoerper, catenin beta 1 S homeolog antikoerper, catenin beta 1 L homeolog antikoerper, CTNNB1 antikoerper, Ctnnb1 antikoerper, ctnnb1 antikoerper, ctnnb1.S antikoerper, ctnnb1.L antikoerper

Hintergrund: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

Gen-ID: 1499

UniProt: P35222

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2