Cyclin E1 Antikörper

Produktnummer ABIN210870

Dieses Anti-Cyclin E1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin E1 in WB, ELISA, IP und ICC. Geeignet für Human.

Kurzübersicht für Cyclin E1 Antikörper (ABIN210870)

Target: Cyclin E1 (CCNE1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Cyclin E1 Antikörper

Spezifität: Recognizes human p52 cyclin E. Cross-reactivity with mouse and rat was not observed.

Aufreinigung: Protein G purified

Immunogen: Recombinant human Cyclin E.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC (10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Proteins were visualized by using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitated cyclin E from 500 μg of human A431 RIPA cell lysate as confirmed by Western blot analysis of the immunoprecipitate using rabbit polyclonal anti-cyclin E. Immunocytochemistry: 10 μg/mL detected cyclin E in 50 % ethanol/50 % acetic acid-fixed human A431 cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: CCNE1 / Cyclin E1

Hintergrund: Name/Gene ID: CCNE1

Synonyms: CCNE1, Cyclin E1, CCNE, Cyclin Es, Cyclin Et, G1/S-specific cyclin-E1, G1/s-specific cyclin e, Cyclin E

Gen-ID: 898

UniProt: P24864

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases