CHEK2 Antikörper
-
- Target Alle CHEK2 Antikörper anzeigen
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
-
Reaktivität
- Human, Maus, Ratte
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser CHEK2 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Spezifität
- Recognizes human CHK2, Mr 67kD. Species cross-reactivity: mouse. Species sequence homology: rat.
- Aufreinigung
- Protein G purified
- Immunogen
-
Mixture of two GST fusion proteins corresponding to amino acids NT, 1-223 and CT 217-543 of human CHK2.
Type of Immunogen: Fusion protein - Isotyp
- IgG1
- Top Product
- Discover our top product CHEK2 Primärantikörper
-
-
- Applikationshinweise
-
Approved: ICC, IP, WB (0.1 - 1 μg/mL)
Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Immunoprecipitation: Detects HK2 from HeLa cells. Reported by an independent lab. Immunocytochemistry: Detects CHK2 in HeLa cells. Reported by an independent lab. Western Blot: 0.1-1.0 μg/mL detects CHK2 in lysates from Jurkat, HeLa, 3T3/A31, and A431 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CHK2 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: J9000: Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/ 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. - Kommentare
-
Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
-
Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
-
- Target
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- Andere Bezeichnung
- CHEK2 / CHK2 (CHEK2 Produkte)
- Synonyme
- CDS1 antikoerper, CHK2 antikoerper, HuCds1 antikoerper, LFS2 antikoerper, PP1425 antikoerper, RAD53 antikoerper, hCds1 antikoerper, fa66f08 antikoerper, wu:fa66f08 antikoerper, zgc:55865 antikoerper, Cds1 antikoerper, HUCDS1 antikoerper, Rad53 antikoerper, Chk2 antikoerper, cds1 antikoerper, chek2 antikoerper, chk2 antikoerper, hucds1 antikoerper, lfs2 antikoerper, pp1425 antikoerper, rad53 antikoerper, checkpoint kinase 2 antikoerper, serine/threonine-protein kinase chk2 antikoerper, checkpoint kinase 2 L homeolog antikoerper, CHEK2 antikoerper, chek2 antikoerper, Chek2 antikoerper, MCYG_07308 antikoerper, chek2.L antikoerper
- Hintergrund
-
Name/Gene ID: CHEK2
Subfamily: CDS1
Family: Protein Kinase
Synonyms: CHEK2, BA444G7, Cds1 homolog, Checkpoint kinase 2, Checkpoint-like protein CHK2, CHK2 checkpoint homolog, CHK2, HCds1, LFS2, RAD53, Rad53 homolog, HCHK2, HuCds1, PP1425 - Gen-ID
- 11200
- UniProt
- O96017
- Pathways
- p53 Signalweg, Apoptose, Zellzyklus
-