CHEK2 Antikörper

Produktnummer ABIN210859

Dieser Maus Monoklonal Antikörper detektiert spezifisch CHEK2 in WB, ICC und IP. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für CHEK2 Antikörper (ABIN210859)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CHEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-CHEK2 Antikörper

Spezifität: Recognizes human CHK2, Mr 67kD. Species cross-reactivity: mouse. Species sequence homology: rat.

Aufreinigung: Protein G purified

Immunogen: Mixture of two GST fusion proteins corresponding to amino acids NT, 1-223 and CT 217-543 of human CHK2.

Type of Immunogen: Fusion protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IP, WB (0.1 - 1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Immunoprecipitation: Detects HK2 from HeLa cells. Reported by an independent lab. Immunocytochemistry: Detects CHK2 in HeLa cells. Reported by an independent lab. Western Blot: 0.1-1.0 μg/mL detects CHK2 in lysates from Jurkat, HeLa, 3T3/A31, and A431 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CHK2 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: J9000: Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/ 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Andere Bezeichnung: CHEK2 / CHK2

Hintergrund: Name/Gene ID: CHEK2
Subfamily: CDS1
Family: Protein Kinase

Synonyms: CHEK2, BA444G7, Cds1 homolog, Checkpoint kinase 2, Checkpoint-like protein CHK2, CHK2 checkpoint homolog, CHK2, HCds1, LFS2, RAD53, Rad53 homolog, HCHK2, HuCds1, PP1425

Gen-ID: 11200

UniProt: O96017

Pathways: p53 Signalweg, Apoptose, Zellzyklus