Der Maus Monoklonal Anti-DYKDDDDK Tag-Antikörper wurde für IF und FACS validiert. Er ist geeignet, DYKDDDDK Tag in Proben von zu detektieren.
Produktnummer ABIN2017574
Kurzübersicht für DYKDDDDK Tag Antikörper (iFluor 488) (ABIN2017574)
Target
DYKDDDDK Tag
Reaktivität
Bitte anfragen
Wirt
Maus
Klonalität
Monoklonal
Konjugat
Dieser DYKDDDDK Tag Antikörper ist konjugiert mit iFluor 488
Applikation
Immunofluorescence (IF), Flow Cytometry (FACS)
Klon
5A8E5
Marke
THE™
Sequenz
DYKDDDDK
Produktmerkmale
THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse is THETM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 488.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed has not been determined. The following concentration ranges are recommended starting points for this product. Immunofluorescent staining: 1-4 μg/mL Flow cytometry: 1-4 μg/mL Other applications: user-optimized
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
Avoid repeated freezing and thawing cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Haltbarkeit
12 months
Target
DYKDDDDK Tag
Substanzklasse
Tag
Hintergrund
The Flag tag is a polypeptide of with the amino acid sequence DYKDDDDK amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.