Topoisomerase II alpha Antikörper (C-Term)

Produktnummer ABIN201464

Der Maus Monoklonal Anti-Topoisomerase II alpha-Antikörper wurde für WB, IHC, FACS, IHC (p), IP und ICC validiert. Er ist geeignet, Topoisomerase II alpha in Proben von Human zu detektieren.

Kurzübersicht für Topoisomerase II alpha Antikörper (C-Term) (ABIN201464)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes a major protein of 170kD which was identified as the human alpha isoform of topoisomerase II. Recognizes a carboxy terminal alpha-isoenzyme specific epitope missing in topoisomerase II beta.

Aufreinigung: Purified

Immunogen: Nuclear protein preparation from the human cell line U937.50.

Type of Immunogen: Cells

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo, ICC (5 - 10 μg/mL), IHC, IHC-P, IP, WB (1 - 10 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Western Blot: 1-10 μg/mL. Immunohistochemistry: 5-10 μg/mL. Strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections. Immunocytochemistry: 5-10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A / Topoisomerase II alpha

Hintergrund: Name/Gene ID: TOP2A
Family: Topoisomerase

Synonyms: TOP2A, DNA topoisomerase II, 170 kD, TOP2, DNA gyrase, DNA topoisomerase 2-alpha, TP2A

Gen-ID: 7153

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases