CTNNB1 Antikörper (C-Term)

Produktnummer ABIN200360

Der Maus Monoklonal Anti-CTNNB1-Antikörper wurde für WB, IHC, IF, IHC (p) und IP validiert. Er ist geeignet, CTNNB1 in Proben von Human, Maus und Huhn zu detektieren.

Kurzübersicht für CTNNB1 Antikörper (C-Term) (ABIN200360)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reaktivität: Human, Maus, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-CTNNB1 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes chicken b-Catenin protein (~92kD). Does not cross-react with the related 83kD g-catenin (plakoglobin) protein. Also specifically detects an additional 120kD protein of unknown identity in extracts derived from A431 cells. This band is not detected in lysates derived from HeLa cells or human fibroblasts. Species cross-reactivity: Human & mouse. Reactivity with rat is likely but not confirmed.

Aufreinigung: Protein A purified

Immunogen: Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken b-Catenin.

Type of Immunogen: Fusion protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IF (5 - 10 μg/mL), IHC, IHC-P, IP, WB (1 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 5-10 μg/mL. Western Blot: 1 μg/mL. Immunoprecipitation: 2-5 μg. Immunohistochemistry (Formalin-fixed and paraffin-embedded tissue): 2-10 μg/mL. Requires heat induced epitope retrieval. Positive control: HeLa, A431 and WI-38 human cell lines.

Kommentare:

Target Species of Antibody: Chicken

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: CTNNB1 / Beta Catenin

Hintergrund: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

Gen-ID: 1499

UniProt: P35222

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2