Perforin 1 Antikörper (PE)

Produktnummer ABIN1981897

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Perforin 1 in ICFC. Er zeigt eine Reaktivität gegenüber Human und Rind (Kuh) und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für Perforin 1 Antikörper (PE) (ABIN1981897)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Perforin 1 Antikörper ist konjugiert mit PE

Applikation: Intracellular Flow Cytometry (ICFC)

Klon: DG9

Produktdetails anti-Perforin 1 Antikörper (PE)

Verwendungszweck: Anti-Perforin PE

Spezifität: The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

Keine Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Human, Bovine

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: purified granules from human YT lymphoma cell line

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-Perforin 1 Antikörper (PE) (ABIN1981897)

Qiu, Chen, Liao, Zhang, Wang, Li, Luo, Fang, Li, Zhou, Shen, Chen, Huang, Cai, Cao, Jiang, Zeng, Chen: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." in: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).

Takeuchi, Inoue, Otani, Yamasaki, Nakamura, Kibata: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." in: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, (2010) (PubMed).

Hersperger, Makedonas, Betts: "Flow cytometric detection of perforin upregulation in human CD8 T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, (2008) (PubMed).

Details zu Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Andere Bezeichnung: Perforin

Hintergrund: Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2

Gen-ID: 5551

UniProt: P14222

Pathways: Apoptose, Caspase Kaskade in der Apoptose