Dieser Perforin 1 Antikörper ist konjugiert mit PE
Applikation
Intracellular Flow Cytometry (ICFC)
Verwendungszweck
Anti-Perforin PE
Spezifität
The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
Keine Kreuzreaktivität
Maus
Kreuzreaktivität (Details)
Human, Bovine
Aufreinigung
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Immunogen
purified granules from human YT lymphoma cell line
PRF1
Reaktivität: Maus
WB, ELISA, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Qiu, Chen, Liao, Zhang, Wang, Li, Luo, Fang, Li, Zhou, Shen, Chen, Huang, Cai, Cao, Jiang, Zeng, Chen: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." in: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).
Takeuchi, Inoue, Otani, Yamasaki, Nakamura, Kibata: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." in: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, (2010) (PubMed).
Hersperger, Makedonas, Betts: "Flow cytometric detection of perforin upregulation in human CD8 T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, (2008) (PubMed).
Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2