Perforin 1 Antikörper (PE)

Produktnummer ABIN1981897

Dieser Maus Monoklonal Antikörper detektiert spezifisch Perforin 1 in ICFC. Es zeigt Reaktivität gegenüber Proben von Human und Rind (Kuh). Es wurde in 3+ Publikationen zitiert.

Kurzübersicht für Perforin 1 Antikörper (PE) (ABIN1981897)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Perforin 1 Antikörper ist konjugiert mit PE

Applikation: Intracellular Flow Cytometry (ICFC)

Klon: DG9

Produktdetails anti-Perforin 1 Antikörper (PE)

Verwendungszweck: Anti-Perforin PE

Keine Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Human, Bovine

Produktmerkmale: The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: purified granules from human YT lymphoma cell line

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze,Protect from prolonged exposure to light

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-Perforin 1 Antikörper (PE) (ABIN1981897)

Qiu, Chen, Liao, Zhang, Wang, Li, Luo, Fang, Li, Zhou, Shen, Chen, Huang, Cai, Cao, Jiang, Zeng, Chen: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." in: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).

Takeuchi, Inoue, Otani, Yamasaki, Nakamura, Kibata: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." in: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, (2010) (PubMed).

Hersperger, Makedonas, Betts: "Flow cytometric detection of perforin upregulation in human CD8 T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, (2008) (PubMed).

Details zu Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Andere Bezeichnung: Perforin

Hintergrund: Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2

Gen-ID: 5551

UniProt: P14222

Pathways: Apoptose, Caspase Kaskade in der Apoptose