CTLA4 Antikörper (PE)

Produktnummer ABIN1981896

Produktdetails anti-CTLA4 Antikörper (PE)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CTLA4 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody BNI3 recognizes an extracellular domain of human CD152 / CTLA4, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human CD152-IgG heavy chain fusion protein

Klon: BNI3

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Kommentare:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CTLA4 Antikörper (PE) (ABIN1981896)

Kraszula, Eusebio, Kupczyk, Kuna, Pietruczuk: "[The use of multi-color flow cytometry for identification of functional markers of nTregs in patients with severe asthma]." in: Pneumonologia i alergologia polska, Vol. 80, Issue 5, pp. 389-401, (2012) (PubMed).

Chin, Chu, Chen, Hsu, Weng, Chu: "Site-directed in vitro immunization leads to a complete human monoclonal IgG4 lambda that binds specifically to the CDR2 region of CTLA-4 (CD152) without interfering the engagement of natural ligands." in: BMC biotechnology, Vol. 7, pp. 51, (2007) (PubMed).

Steiner, Moosig, Csernok, Selleng, Gross, Fleischer, Bröker: "Increased expression of CTLA-4 (CD152) by T and B lymphocytes in Wegener's granulomatosis." in: Clinical and experimental immunology, Vol. 126, Issue 1, pp. 143-50, (2001) (PubMed).

Steiner, Waase, Rau, Dietrich, Fleischer, Bröker: "Enhanced expression of CTLA-4 (CD152) on CD4+ T cells in HIV infection." in: Clinical and experimental immunology, Vol. 115, Issue 3, pp. 451-7, (1999) (PubMed).

Details zu CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Andere Bezeichnung: CD152 (CTLA4 Produkte)

Hintergrund: Cytotoxic T-lymphocyte associated protein 4,CD152 / CTLA-4 is a homodimeric transmembrane protein similar to CD28 and binding the same ligands, i.e. CD80 (B7.1) and CD86 (B7.2), but with higher affinity. Unlike CD28 with important costimulating functions, CD152 acts as an important inhibitory receptor essential for modulation of the immune system. CD152 / CTLA-4 becomes transiently expressed on activated T cells and its malfunction can cause autoimmune diseases, such as insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, or thyroid-associated orbitopathy.,CTLA4, GSE, GRD4

Gen-ID: 1493

UniProt: P16410

Pathways: Cancer Immune Checkpoints