Sonic Hedgehog Antikörper (AA 199-437)

Produktnummer ABIN6757522

Dieses Anti-Sonic Hedgehog-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von Sonic Hedgehog in WB und ELISA. Geeignet für Maus.

Kurzübersicht für Sonic Hedgehog Antikörper (AA 199-437) (ABIN6757522)

Target: Sonic Hedgehog (SHH)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Sonic Hedgehog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Sonic Hedgehog Antikörper

Bindungsspezifität: AA 199-437

Spezifität: Recognizes mouse Shh C-terminal peptide. In direct ELISA, does not cross-react with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide or rmShh N-terminal peptide.

Aufreinigung: Protein G purified

Immunogen: Purified, E. coli-derived, recombinant mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide, aa 199-437).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and ELISA. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for rmShh C-terminal peptide is approximately 1 ng/well. Western Blot: 1-2 μg/mL with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for recombinant mouse Shh C-terminal peptide is approximately 0.5 ng/lane under non-reducing and reducing conditions.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: Sonic Hedgehog / SHH

Hintergrund: Name/Gene ID: SHH
Subfamily: Cysteine C46
Family: Protease

Synonyms: SHH, HHG-1, HLP3, Sonic hedgehog, Sonic hedgehog homolog, MCOPCB5, Sonic hedgehog protein, HHG1, HPE3, SMMCI, TPT, TPTPS

Gen-ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development