CDC25A Antikörper (Ser75)

Produktnummer ABIN197370

Der Kaninchen Polyklonal anti-CDC25A Antikörper (ABIN197370) detektiert spezifisch CDC25A in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für CDC25A Antikörper (Ser75) (ABIN197370)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC25A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CDC25A Antikörper

Bindungsspezifität: Ser75

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. cdc25A antibody AP02684PU detects endogenous levels of total cdc25A protein.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human cdc25A around the phosphorylation site of serine 75 (M-G-Sp-S-E).

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1: 500-1: 1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Andere Bezeichnung: CDC25A

Hintergrund: Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1

Gen-ID: 993

NCBI Accession: NP_001780

UniProt: P30304

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase