NFKBIB Antikörper (pSer23)

Produktnummer ABIN197074

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch NFKBIB in IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für NFKBIB Antikörper (pSer23) (ABIN197074)

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFKBIB Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NFKBIB Antikörper

Bindungsspezifität: pSer23

Spezifität: IκB-β antibody detects endogenous levels of total IκB-β protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human IkappaB- beta around the phosphorylation site of serine 23 (L-G-SP-L-G ).

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu NFKBIB

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Andere Bezeichnung: NFKBIB / IKBB

Hintergrund: Three major forms of IkB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkB-alpha and IkB-beta appear to be the major regulatory forms of IkB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkB and block nuclear import by masking the nuclear localization sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkB. Immunoblotting easily detects the hyper-phosphorylated forms of IkB-alpha, but not phosphorylated IkB-beta. Interestingly, IkB-alpha and IkB-beta mediate different NFkB responses. IkB-alpha appears to control more transient activation of NFkB in response to an inducer, while IkB-beta controls a persistent response. Bcl-3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkB is confirmed by immunoblotting.Synonyms: I-kappa-B-beta, IkB-B, IkB-beta, IkappaBbeta, NF-kappa-B inhibitor beta, TR-interacting protein 9, TRIP9, Thyroid receptor-interacting protein 9

Gen-ID: 4793

NCBI Accession: NP_001001716

UniProt: Q15653

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades