NCOA1 Antikörper (pTyr529)

Produktnummer ABIN196876

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NCOA1 in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für NCOA1 Antikörper (pTyr529) (ABIN196876)

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCOA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NCOA1 Antikörper

Bindungsspezifität: pTyr529

Spezifität: The antibody detects endogenous levels of Src only when phosphorylated at Tyrosine 529.

Aufreinigung: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu NCOA1

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Andere Bezeichnung: SRC1

Hintergrund: Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

Gen-ID: 6714

NCBI Accession: NP_005408

UniProt: P12931

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha