Der Kaninchen Polyklonal anti-RPS6KA1 Antikörper wird verwendet zum Nachweis von RPS6KA1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IF und IHC (p).
This antibody detects endogenous levels of p90RSK only when phosphorylated at Threonine 348.
Aufreinigung
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from mouse p90RSK around the phosphorylation site of threonine 348 (S-R-TP-P-R).
RPS6KA1
Reaktivität: Human
ELISA, IHC
Wirt: Maus
Monoclonal
10B1D7A9
unconjugated
Applikationshinweise
Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody (in aliquots) at -20 °C.
Target
RPS6KA1
(Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))