PDPK1 Antikörper (pSer241)

Produktnummer ABIN196616

Produktdetails anti-PDPK1 Antikörper

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Bindungsspezifität: pSer241

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDPK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Andere Bezeichnung: PDPK1 (PDPK1 Produkte)

Synonyme: pdpk1 antikoerper, MGC82080 antikoerper, PDK1 antikoerper, PDPK2 antikoerper, PRO0461 antikoerper, Pdk1 antikoerper, zgc:153787 antikoerper, 3'-phosphoinositide-dependent protein kinase 1 antikoerper, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 antikoerper, ATPDK1 antikoerper, AtPDK1 antikoerper, T32M21.110 antikoerper, T32M21_110 antikoerper, zgc:77318 antikoerper, 3-phosphoinositide dependent protein kinase 1 antikoerper, 3-phosphoinositide dependent protein kinase 1 L homeolog antikoerper, 3-phosphoinositide dependent protein kinase-1 antikoerper, 3-phosphoinositide dependent protein kinase 1a antikoerper, 3-phosphoinositide-dependent protein kinase 1 antikoerper, 3'-phosphoinositide-dependent protein kinase 1 antikoerper, 3-phosphoinositide dependent protein kinase 1b antikoerper, PDPK1 antikoerper, pdpk1.L antikoerper, pdpk1 antikoerper, Pdpk1 antikoerper, pdpk1a antikoerper, LOC100380750 antikoerper, pdk-1 antikoerper, PDK1 antikoerper, pdpk1b antikoerper

Hintergrund: Synonyms: 3-phosphoinositide-dependent proteinkinase 1, PDK-1, PDK1, PDPK1, hPDK1

Gen-ID: 5170

NCBI Accession: NP_002604

UniProt: O15530

Pathways: PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals