PAX6 Antikörper (Internal Region)

Produktnummer ABIN190794

Dieses Anti-PAX6-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von PAX6 in WB, ELISA und FACS. Geeignet für Human und Maus.

Kurzübersicht für PAX6 Antikörper (Internal Region) (ABIN190794)

Target: PAX6 (Paired Box 6 (PAX6))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PAX6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-PAX6 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: PAX6 (internal)

Sequenz: REEKLRNQRR QASN

Spezifität: This antibody is expected to recognize all reported isoforms: NP_000271.1, NP_001595.2, NP_001297088.1, NP_001355839.1, NP_001355840.1, NP_001355841.1, NP_001355844.1, NP_001355847.1, NP_001355849.1, NP_001355850.1, NP_001355851.1, NP_001355857.1, NP_0013

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-REEKLRNQRRQASN, from the internal region of the protein sequence according to NP_000271.1, NP_001595.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 26 kDa band observed in lysates of cell lines U251 and RAW264.7 and in nuclear lysates of cell lines K562 and HeLa, all successfully blocked by incubation with the immunising peptide (calculated MW of 24.8 kDa according to Human NP_001

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Andere Bezeichnung: PAX6

Hintergrund: PAX6, paired box gene 6 (aniridia, keratitis), AN, AN2, D11S812E, MGC17209, MGDA, WAGR, paired box gene 6, paired box homeotic gene 6 (aniridia, keratitis), paired box homeotic gene-6

Gen-ID: 5080, 18508, 25509

NCBI Accession: NP_000271, NP_001595

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis