ILF2 Antikörper (Internal Region)

Produktnummer ABIN190786

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ILF2 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für ILF2 Antikörper (Internal Region) (ABIN190786)

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ILF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Güteklasse: Verified

Produktdetails anti-ILF2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ILF2 / NF45

Sequenz: EKAYEKPPEK KEG

Spezifität: This antibody is expected to recognize both reported isoforms (NP_004506.2, NP_001254738.1).

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKAYEKPPEKKEG, from the internal region (near the C Terminus) of the protein sequence according to NP_004506.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 43 kDa band observed in Human Tonsil and Thymus lysates (calculated MW of 43.1 kDa according to NP_004506.2). Recommended concentration: 0.03-0.1 μg/mL. This antibody has been successfully used in WB in the following papers: Ye J et al

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunoprecipitation: This antibody was confirmed by a customer in a special IP procedure called PAR-CLIP (see for method PMID: 20644507). This data is from a previous batch, not on sale.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-ILF2 Antikörper (ABIN190786)

Shamanna, Hoque, Peery, Mathews: "Induction of p53, p21 and apoptosis by silencing the NF90/NF45 complex in human papilloma virus-transformed cervical carcinoma cells." in: Oncogene, Vol. 32, Issue 43, pp. 5176-85, (2013) (PubMed).

Shamanna, Hoque, Lewis-Antes, Azzam, Lagunoff, Peery, Mathews: "The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining." in: Molecular and cellular biology, Vol. 31, Issue 23, pp. 4832-43, (2011) (PubMed).

Details zu ILF2

Target: ILF2 (Interleukin Enhancer Binding Factor 2, 45kDa (ILF2))

Andere Bezeichnung: ILF2

Hintergrund: ILF2, NF45, interleukin enhancer binding factor 2, 45 kDa , MGC8391, PRO3063 , interleukin enhancer binding factor 2, nuclear factor of activated T-cells, 45- kDa

Gen-ID: 3608, 67781, 310612

NCBI Accession: NP_004506, NP_001254738