PRTN3 Antikörper

Produktnummer ABIN190718

Der Kaninchen Polyklonal anti-PRTN3 Antikörper wird verwendet zum Nachweis von PRTN3 in Proben von Agrostis stolonifera, Nicotiana tabacum, Picea abies, Potato, Red pepper, Tomato und Vitis vinifera. Er wurde validiert für WB, IL und Co-IP.

Kurzübersicht für PRTN3 Antikörper (ABIN190718)

Target: PRTN3 (Proteinase 3 (PRTN3))

Reaktivität: Agrostis stolonifera, Nicotiana tabacum, Picea abies, Potato, Red pepper, Tomato, Vitis vinifera

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRTN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL), Co-Immunoprecipitation ( Co-IP)

Produktdetails anti-PRTN3 Antikörper

Verwendungszweck: Anti-PR-3 / CHN | Class I chitinase

Spezifität: Antibody is recognizing closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa).

Kreuzreaktivität (Details): Agostis stolonifera cv. 'Penncross', Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Not reactive in: Cicer arietinum

Homologie: Arabidopsis thaliana,Manihot esculenta,Zea mays

Aufreinigung: Protein G purified

Immunogen: Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 8 μg/mL (WB)

Kommentare:

Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Details zu PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Andere Bezeichnung: PR-3

Hintergrund:

Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

Additional information: Antibody is recognizing closely related tobacco class I isoforms: endochitinase A CHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

Molekulargewicht: 35, 34 | 32 and 34 kDa

Gen-ID: 107759005

UniProt: P08252, P24091