PRTN3 Antikörper

Produktnummer ABIN190718

Der Kaninchen Polyklonal Anti-PRTN3-Antikörper wurde für WB validiert. Er ist geeignet, PRTN3 in Proben von Nicotiana tabacum und Potato zu detektieren.

Kurzübersicht für PRTN3 Antikörper (ABIN190718)

Target: PRTN3 (Proteinase 3 (PRTN3))

Reaktivität: Nicotiana tabacum, Potato

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRTN3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PRTN3 Antikörper

Spezifität: Antibody is recognizing closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa).

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 35, 34 / 32 and 34 kDa

Aufreinigung: affinity purified

Immunogen: purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990, van Buuren et al., 1992): 1) Chitinase A (CHN A) P08252 encoded by gene chn48 derived from the N. tomentosiformis ancestor of tobacco. 2) Chitinase B (CHN B) P24091 encoded by gene chn50 derived from the N. sylvestris ancestor of tobacco.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 8 ug/mL with standard ECL (WB).

Kommentare:

Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL of sterile water.

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Details zu PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Andere Bezeichnung: PR-3

Hintergrund: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

Molekulargewicht: expected: 35 kDa, 34 kDa, apparent: 32 and 34 kDa

UniProt: P08252, P24091