GCLC Antikörper

Produktnummer ABIN190704

Dieses Anti-GCLC-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GCLC in WB und IL. Geeignet für Mais (Zea mays), Tomato, Nicotiana tabacum und Arabidopsis thaliana. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für GCLC Antikörper (ABIN190704)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reaktivität: Mais (Zea mays), Tomato, Nicotiana tabacum, Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GCLC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL)

Produktdetails anti-GCLC Antikörper

Spezifität: The antibody recognizes closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)

Kreuzreaktivität (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: dicots including:Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 50 kDa
This antibody can be used as a marker of vacuolar contents.

Aufreinigung: affinity purified

Immunogen: KLH-conjugated synthetic peptide derived from Zea mays gamma-ECS Q8W4W3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1: 5000 with standard ECL (WB), 1: 5000 (IL)

Kommentare:

antibodies has been used in immunolocalization on Arabidopsis thaliana

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water.

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-GCLC Antikörper (ABIN190704)

Sobrino-Plata, Carrasco-Gil, Abadía, Escobar, Álvarez-Fernández, Hernández: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).

Keefe, Hinz, Meins: "The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves." in: Planta, Vol. 182, Issue 1, pp. 43-51, (2013) (PubMed).

Details zu GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Andere Bezeichnung: Gamma-ECS

Hintergrund: AGI Code: At4g23100
Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

Molekulargewicht: 50 kDa

UniProt: Q8W4W3

Pathways: Cell RedoxHomeostasis