UCHL3 Antikörper (C-Term)

Produktnummer ABIN1882147

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch UCHL3 in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für UCHL3 Antikörper (C-Term) (ABIN1882147)

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UCHL3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB4187

Produktdetails anti-UCHL3 Antikörper

Bindungsspezifität: AA 195-225, C-Term

Homologie: B, M, Pig, Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-UCHL3 Antikörper (ABIN1882147)

Henderson, Vij, Zeitlin: "Ubiquitin C-terminal hydrolase-L1 protects cystic fibrosis transmembrane conductance regulator from early stages of proteasomal degradation." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11314-25, (2010) (PubMed).

Sun, Ying, Li, Shang, He, Chen, Cheng, Zhou: "Role of UCH-L1/ubiquitin in acute testicular ischemia-reperfusion injury." in: Biochemical and biophysical research communications, Vol. 366, Issue 2, pp. 539-44, (2008) (PubMed).

Saito, Iida, Sekine, Kawauchi, Higuchi, Ogawa, Nakamura: "Catalog of 680 variations among eight cytochrome p450 ( CYP) genes, nine esterase genes, and two other genes in the Japanese population." in: Journal of human genetics, Vol. 48, Issue 5, pp. 249-70, (2003) (PubMed).

Wilkinson, Lee, Deshpande, Duerksen-Hughes, Boss, Pohl: "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase." in: Science (New York, N.Y.), Vol. 246, Issue 4930, pp. 670-3, (1989) (PubMed).

Details zu UCHL3

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Andere Bezeichnung: UCHL3

Hintergrund: Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.

Molekulargewicht: 26183

NCBI Accession: NP_001257881, NP_005993

UniProt: P15374

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation