Cyclin B1 Antikörper (pSer35)

Produktnummer ABIN1881155

Der Kaninchen Polyklonal Anti-Cyclin B1-Antikörper wurde für WB und DB validiert. Er ist geeignet, Cyclin B1 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für Cyclin B1 Antikörper (pSer35) (ABIN1881155)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB)

Klon: RB42141

Produktdetails anti-Cyclin B1 Antikörper

Bindungsspezifität: pSer35

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1100. DB: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-Cyclin B1 Antikörper (ABIN1881155)

Kreis, Sanhaji, Krämer, Sommer, Rödel, Strebhardt, Yuan: "Restoration of the tumor suppressor p53 by downregulating cyclin B1 in human papillomavirus 16/18-infected cancer cells." in: Oncogene, Vol. 29, Issue 41, pp. 5591-603, (2010) (PubMed).

van Zon, Ogink, ter Riet, Medema, te Riele, Wolthuis: "The APC/C recruits cyclin B1-Cdk1-Cks in prometaphase before D box recognition to control mitotic exit." in: The Journal of cell biology, Vol. 190, Issue 4, pp. 587-602, (2010) (PubMed).

Harley, Allan, Sanderson, Clarke: "Phosphorylation of Mcl-1 by CDK1-cyclin B1 initiates its Cdc20-dependent destruction during mitotic arrest." in: The EMBO journal, Vol. 29, Issue 14, pp. 2407-20, (2010) (PubMed).

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: CCNB1

Hintergrund: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].

Molekulargewicht: 48337

NCBI Accession: NP_114172

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase