Keratin Acidic (AE1) Antikörper

Produktnummer ABIN187689

Der Maus Monoklonal anti-Keratin Acidic Antikörper (Klon AE-1) (ABIN187689) detektiert spezifisch Keratin Acidic in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Huhn, Rind (Kuh) und Kaninchen.

Kurzübersicht für Keratin Acidic (AE1) Antikörper (ABIN187689)

Target: Keratin Acidic (AE1)

Reaktivität: Human, Ratte, Maus, Huhn, Rind (Kuh), Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: AE-1

Produktdetails

Spezifität: AE-1 detects in immunoblots distinct bands of 40 kDa, 45 kDa, 46 kDa, 48 kDa, 50 kDa, (50' kDa), 51 kDa, 55 kDa, and 56.5 kDa with all Ip's lower than 5.7. These MW's are believed to correspond with keratin 19, 18, 17, 16, 15, 14, 13, 12 and 10, respectively. Species Reactivity: Human, bovine, chicken, mouse, rabbit, and rat. Others not tested.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: (Formalin-fixed / Paraffin-embedded sections) Use at 1:150-1:200 for 30 min. at 37 °C. Higher dilutions can be used if sections are incubated at 4 °C overnight. Western blot: Use at 1:100. The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 250 µg purified IgG at 1 mg/ml with 0.1% sodium azide.

Lagerung: 4 °C

Antigendetails

Target: Keratin Acidic (AE1)

Andere Bezeichnung: Cytokeratin (Epithelial type I)

Hintergrund: This antibody recognizes most of the acidic (type I) keratins. Each epithelium contains at least one acidic keratin. AE-1 is broadly reactive with almost all epithelial and their neoplasms. The antibody exhibits heterogenous staining within a given epithelium, the detailed staining pattern varies depending on the differentiated and/or growth state of the individual cells. This antibody together with MC-976 (clone AE-3) has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelial development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma.