Caspase 2 Antikörper

Produktnummer ABIN187544

Der Ratte Monoklonal anti-Caspase 2 Antikörper wird verwendet zum Nachweis von Caspase 2 in Proben von Human, Maus, Ratte und Hund. Er wurde validiert für WB und IP.

Kurzübersicht für Caspase 2 Antikörper (ABIN187544)

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Caspase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 11B4

Produktdetails anti-Caspase 2 Antikörper

Spezifität: Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51 kDa (procaspase-2), ~32 kDa and ~18 kDa (cleavage products) by Western blot. Species Reactivity: Human, mouse, rat, monkey and dog. Others not tested.

Immunogen: p19 fragment of recombinant human Caspase-2.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation Western blot: Use at 2 µg/mL. The optimal dilution for a specific application should be determined by the researcher. Figure (left column): Detection of procaspase-2 (p51), an intermediate cleavage product of caspase-2 (p32) and activated caspase-2 (p19) in FDC-P1 cells (mouse IL-3 dependent promyelocytic line) during growth factor withdrawal induced apoptosis. Activation of caspase-2 is inhibited in the presence of the caspase inhibitor Z-VAD-FMK (AB-001). Figures (above): Western blot analysis of cell lysates from human and mouse cell lines with clone 11B4 (MC-498). Clone 11B4 demonstrates its ability to recognize an approximately 51 kDa protein that corresponds to endogenous procaspase-2. Hsp-70 was used as an equal loading control, and -/- caspase-2 cells were used as a negative control.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 µL of protein G affinity-purified monoclonal antibody at 1 mg/mL in PBS containing 0.02% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu Caspase 2

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Andere Bezeichnung: Caspase-2

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Neurotrophin Signalübertragung