GZMB Antikörper

Produktnummer ABIN187356

Dieser Anti-GZMB-Antikörper ist ein Maus-Monoklonal-Antikörper zum Nachweis von GZMB in WB, FACS, IF, IHC (p) und IHC (fro). Geeignet für Human.

Kurzübersicht für GZMB Antikörper (ABIN187356)

Target: GZMB (Granzyme B (GZMB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GZMB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: B18-1

Produktdetails anti-GZMB Antikörper

Spezifität: Recognizes human granzyme B. Species Reactivity: Human. Others not tested. Positive Control: Human CD8+ T cells.

Reinheit: 95 % as determined by SDS-PAGE.

Immunogen: Recombinant human granzyme B (aa 7-227)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry Immunocytochemistry Immunoprecipitation Immunohistochemistry: (frozen sections and paraffin sections) Western blot: Suggested dilution of 1 µg/mL The optimal dilution for a specific application should be determined by the researcher. Figure A: Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 µg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. Figure B: Detection of granzyme B in a human CD8+ T cell clone. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min., then in acetone at -20 °C for 30 seconds. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hour at room temperature (RT) with 20 µg/mL of clone B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 100 µg of liquid purified monoclonal antibody at 1 mg/mL in PBS with 0.02% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu GZMB

Target: GZMB (Granzyme B (GZMB))

Hintergrund: Granzyme B is a 27 kDa serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and, assisted by perforin, enters the target cell. In the target, granzyme B processes and activates various pro-caspases, inducing apoptosis in the target cell.

Pathways: Apoptose, Caspase Kaskade in der Apoptose