FASL Antikörper

Produktnummer ABIN187307

Der Maus Monoklonal anti-FASL Antikörper wird verwendet zum Nachweis von FASL in Proben von Human. Er wurde validiert für FACS, IP, IHC (p), Func, IHC (fro) und EIA.

Kurzübersicht für FASL Antikörper (ABIN187307)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FASL Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 2C101

Produktdetails anti-FASL Antikörper

Spezifität: This antibody is specific for the extracellular domain of human Fas Ligand. Species Reactivity: Human. Others not tested.

Immunogen: Recombinant human FasL (extracellular domain)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Functional Activity: Inhibits apoptosis in anti-CD3 stimulated Jurkat T-cells ( 0.5 ~ 5 µg/mL) suitable. Excellent for neutralization. Flow cytometry: 1 x 106 H9 T-cells stained with 1 µg/mL antibody, followed by biotinylated goat anti-mouse IgG and streptavidin-PE. Immunocytochemistry Suggested starting dilution is >1:100 The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 100 µg of 1 mg/mL affinity-purified monoclonal antibody. Prepared in 0.15M PBS, pH 7.2 with protein stabilizer. Purity 95% by SDS-PAGE.

Lagerung: 4 °C

Details zu FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Andere Bezeichnung: CD178 / Fas Ligand

Hintergrund: Fas (CD95/APO-1) Ligand (FasL) is a 43 kDa type II membrane protein and member of the tumor necrosis factor family. Human FasL can be released from cells as a 26 kDa soluble protein upon cleavage by metalloproteinases. FasL is expressed on various cells including T cells, NK cells, Monocytes, Sertoli cells and tumor cells. Interaction of FasL with its receptor triggers rapid apoptosis. FasL-induced apoptosis is involved in T cell receptor/anti-CD3 induced cell death, cytotoxic T cell activity, maintainence of immune privilege, immune escape of tumor cells and viral hepatitis. Malfunction of the Fas system causes hyperplasia.

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity