FASL Antikörper

Produktnummer ABIN187289

Der Ratte Monoklonal Anti-FASL-Antikörper wurde für WB, FACS und IF validiert. Er ist geeignet, FASL in Proben von Maus zu detektieren.

Kurzübersicht für FASL Antikörper (ABIN187289)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser FASL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: H11

Produktdetails anti-FASL Antikörper

Spezifität: Species Reactivity: Mouse. Others not tested.

Reinheit: > 95 % by SDS-PAGE.

Immunogen: Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein (CD95L, APO-1L, CD178).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: Use anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). Flow Cytometry: Use Anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). [ Note: Activated T cells do not express high levels of surface Fas Ligand.] Western Blot: Excellent for this application. Use 2 µg/mL. Use unlabeled (Cat. No. MC-106) or biotin-labeled (Cat. No. MC-126). The optimal dilution for a specific application should be determined by the researcher. Figure 1. Immunocytochemistry: Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min, then in acetone at -20 °C for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at RT with 20 µg/mL of biotinylated H11 antibody (MC-126) in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min. Slides were washed again in PBS and visualized using a fluorescence microscope. Method: FasL transfected 293T cells (5x105) were incubated on ice for 30 min in 50 µL FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1 µg of FITC-labeled H11 antibody (MC-136). After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 µL of 1 mg/mL antibody in PBS with 0.02% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Andere Bezeichnung: CD178 / Fas Ligand

Hintergrund: Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. FasL is released by cleavage by metalloproteinases. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced cell death of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and in certain tumor cells.

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity