Cytokeratin 18 Antikörper (AA 1-159)

Produktnummer ABIN1862828

Der Kaninchen Polyklonal Anti-Cytokeratin 18-Antikörper wurde für WB, IHC, IP und ICC validiert. Er ist geeignet, Cytokeratin 18 in Proben von Schwein zu detektieren.

Kurzübersicht für Cytokeratin 18 Antikörper (AA 1-159) (ABIN1862828)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cytokeratin 18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Cytokeratin 18 Antikörper

Bindungsspezifität: AA 1-159

Verwendungszweck: Polyclonal Antibody to Cytokeratin 18 (CK18)

Spezifität: The antibody is a rabbit polyclonal antibody raised against CK18. It has been selected for its ability to recognize CK18 in immunohistochemical staining and western blotting.

Kreuzreaktivität: Human, Schwein

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB231Po01Recombinant Cytokeratin 18 (CK18)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: CK18

Hintergrund: KRT18, CYK18, K18, Keratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18

Pathways: Apoptose, Caspase Kaskade in der Apoptose