SIRT1 Antikörper (Internal Region)

Produktnummer ABIN185702

Der Ziege Polyklonal Anti-SIRT1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, SIRT1 in Proben von Maus zu detektieren.

Kurzübersicht für SIRT1 Antikörper (Internal Region) (ABIN185702)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SIRT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Güteklasse: Verified

Produktdetails anti-SIRT1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: SIRT1

Sequenz: EITEKPPRTQ KE

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EITEKPPRTQKE, from the internal region of the protein sequence according to NP_036370.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 110 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 80.3 kDa according to Mouse NP_062786.1). Preliminary testing also showed a band at approx 120x kDa in Fetal Mouse Brain lysate. This molecular weight is routinely o

Peptide ELISA: antibody detection limit dilution 1:64000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Andere Bezeichnung: SIRT1

Hintergrund: SIRT1 , sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , RP11-57G10.3, SIR2L1 , OTTHUMP00000060745, SIR2alpha, sir2-like 1, sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1, sirtuin 1, sirtui

Gen-ID: 23411, 93759, 309757

NCBI Accession: NP_036370

Pathways: MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling