OGFR Antikörper (C-Term)

Produktnummer ABIN185551

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch OGFR in WB, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für OGFR Antikörper (C-Term) (ABIN185551)

Target: OGFR (Opioid Growth Factor Receptor (OGFR))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser OGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-OGFR Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: OGFR

Sequenz: QDAEVESSAK SGK

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QDAEVESSAKSGK, from the C Terminus of the protein sequence according to NP_031372.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 75 kDa band observed in nuclear cell lysates of HEK293 and A431 (calculated MW of 73.3 kDa according to NP_031372.2) Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu OGFR

Target: OGFR (Opioid Growth Factor Receptor (OGFR))

Andere Bezeichnung: OGFR

Hintergrund: OGFR, opioid growth factor receptor, HGNC:15768, 7-60 protein, zeta-type opioid receptor, met-enkephalin receptor

Gen-ID: 11054

NCBI Accession: NP_031372