PDE4D Antikörper (C-Term)

Produktnummer ABIN185530

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch PDE4D in WB, ELISA, IHC und EIA. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für PDE4D Antikörper (C-Term) (ABIN185530)

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PDE4D Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Güteklasse: Verified

Produktdetails anti-PDE4D Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: PDE4D

Sequenz: QPEACVIDDR SPDT

Spezifität: This antiobody is expected to recognize all reported isoforms.

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_001184151.1, NP_001184150.1, NP_006194.2, NP_001098101.1, NP_001184152.1, NP_001159371.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 70+80+90 kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. Calculated

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol. 2010 Jan,47(4):678-84., PMID: 19939455.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-PDE4D Antikörper (ABIN185530)

Bazhin, Kahnert, Kimpfler, Schadendorf, Umansky: "Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells." in: Molecular immunology, Vol. 47, Issue 4, pp. 678-84, (2010) (PubMed).

Details zu PDE4D

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Andere Bezeichnung: PDE4D

Hintergrund: PDE4D, phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila), HGNC:8783, DPDE3, HSPDE4D, PDE4DN2, STRK1, cAMP-specific phosphodiesterase 4D, cAMP-specific phosphodiesterase PDE4D6, dunce-like phosphodiesterase E3, phosphodie

Gen-ID: 5144

NCBI Accession: NP_001184151, NP_001184150, NP_006194, NP_001098101, NP_001184152, NP_001159371

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling