Caveolin-1 Antikörper (Internal Region)

Produktnummer ABIN185421

Dieses Anti-Caveolin-1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Caveolin-1 in WB, IHC, ELISA, IF und FACS. Geeignet für Human.

Kurzübersicht für Caveolin-1 Antikörper (Internal Region) (ABIN185421)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Caveolin-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Caveolin-1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Caveolin 1

Sequenz: DELSEKQVYD AH

Spezifität: This antibody is expected to recognize both reported isoforms (NP_001744.2, NP_001166366.1). Reported variants represent identical protein: NP_001166367.1, NP_001166368.1, NP_001166366.1. No cross-reactivity expected to Caveolin 2 and Caveolin 3

Kreuzreaktivität: Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DELSEKQVYDAH, from the internal region of the protein sequence according to NP_001744.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 22 kDa band observed in lysates of cell lines A431 and A549 and in Human Heart lysates (calculated MW of 20.5 kDa according to NP_001744.2). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at room temperature. Pre

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of A431 cells and in the cytoplasm and Golgi of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analy

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Andere Bezeichnung: CAV1

Hintergrund: CAV1, caveolin 1, caveolae protein, 22 kDa , HGNC:1527, CAV, MSTP085, VIP21, caveolae protein, 22-kD, caveolin 1, caveolin 1 caveolae protein, 22kD, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32, BSCL3, CGL3, MSTP08

Gen-ID: 857, 12389, 25404

NCBI Accession: NP_001744, NP_001166366

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals