BID Antikörper (C-Term)

Produktnummer ABIN185380

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch BID in WB, ELISA und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für BID Antikörper (C-Term) (ABIN185380)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BID Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-BID Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: BID

Sequenz: TYVRSLARNG MD

Spezifität: This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1, NP_001187.1, NP_932071.1)

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TYVRSLARNGMD, from the C Terminus of the protein sequence according to NP_932070.1, NP_001187.1, NP_932071.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 26+24 kDa bands observed in A431 cell lysates (calculated MW of 26.8 kDa according to NP_932070.1 and 22.0 kDa according to NP_001187.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Andere Bezeichnung: BID

Hintergrund: BID, BH3 interacting domain death agonist , HGNC:1050, MGC15319, MGC42355 , BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist, FP497, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, OTTHUMP00000196197, desmocoll

Gen-ID: 637

NCBI Accession: NP_932070, NP_001187, NP_932071

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity