HNF4A Antikörper (N-Term)

Produktnummer ABIN185378

Der Ziege Polyklonal Anti-HNF4A-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, HNF4A in Proben von Human zu detektieren.

Kurzübersicht für HNF4A Antikörper (N-Term) (ABIN185378)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HNF4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-HNF4A Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: HNF4A

Sequenz: RLSKTLVDMD MADY

Spezifität: This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1, NP_000448.3, NP_849181.1 resp.).

Kreuzreaktivität: Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1, NP_000448.3, NP_849181.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2 μg/mL.

Western Blot: Approx 55 kDa and 48 kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8 kDa according to Human NP_000448.3 , 46.6 kDa according to Human NP_849181.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Andere Bezeichnung: HNF4A

Hintergrund: HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14, FLJ39654, HNF4alpha, MODY, MODY1, HNF4alpha10/11/12, hepatic nuclear factor 4 alpha

Gen-ID: 3172

NCBI Accession: NP_849180, NP_000448, NP_849181

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process