TRIM5 Antikörper (N-Term)

Produktnummer ABIN184983

Dieses Anti-TRIM5-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von TRIM5 in WB, ELISA und IHC. Geeignet für Human.

Kurzübersicht für TRIM5 Antikörper (N-Term) (ABIN184983)

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TRIM5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-TRIM5 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: TRIM5 / RNF88

Sequenz: ASGILVNVKE EVTC

Spezifität: This antibody is expected to recognise all three human isoforms of this protein.

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1, NP_149083.1, NP_149084.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 3 μg/mL.

Western Blot: Approx 55 and 37 kDa bands observed in Human Thymus lysates (calculated MW of 56.3 kDa according to NP_149023.1 and of 37.7 kDa according to NP_149084.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Andere Bezeichnung: TRIM5

Hintergrund: TRIM5, RNF88, tripartite motif-containing 5, TRIM5alpha, tripartite motif protein 5, tripartite motif protein TRIM5, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha

Gen-ID: 85363

NCBI Accession: NP_149023, NP_149083, NP_149084

Pathways: Activation of Innate immune Response