KIF5B Antikörper (C-Term)

Produktnummer ABIN184774

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch KIF5B in WB, ELISA und IF. Er zeigt eine Reaktivität gegenüber Human und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für KIF5B Antikörper (C-Term) (ABIN184774)

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KIF5B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-KIF5B Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Kinesin 1 / UKHC

Sequenz: QPVAVRGGGG KQV

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Immunofluorescence:This antibody has been successfully used in IF on Human: Connell et al. (20

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-KIF5B Antikörper (ABIN184774)

Connell, Allison, Rodger, Pearson, Zlamalova, Reid: "ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic." in: Cellular and molecular life sciences : CMLS, Vol. 77, Issue 13, pp. 2641-2658, (2020) (PubMed).

Robinson, Evans, Briggs, Ramalho, Hume: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).

Yasuda, Clatterbuck-Soper, Jackrel, Shorter, Mili: "FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination." in: The Journal of cell biology, Vol. 216, Issue 4, pp. 1015-1034, (2017) (PubMed).

Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, (2010) (PubMed).

Gupta, Palmer, Spence, Hudson, Stephens: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, (2008) (PubMed).

Details zu KIF5B

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Andere Bezeichnung: KIF5B

Hintergrund: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain

Gen-ID: 3799

NCBI Accession: NP_004512

Pathways: Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization