WNT4 Antikörper (Internal Region)

Produktnummer ABIN184715

Der Ziege Polyklonal Anti-WNT4-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, WNT4 in Proben von Human zu detektieren.

Kurzübersicht für WNT4 Antikörper (Internal Region) (ABIN184715)

Target: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser WNT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-WNT4 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: WNT4

Sequenz: SNWLYLAKLS SVGS

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SNWLYLAKLSSVGS, from the internal region of the protein sequence according to NP_110388.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 40+35 kDa band observed in lysates of cell line Jurkat (calculated MW of 39.1 kDa according to NP_110388.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: U937

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu WNT4

Target: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Andere Bezeichnung: WNT4

Hintergrund: WNT4, wingless-type MMTV integration site family, member 4, WNT-4, WNT-4 protein precursor, RP1-224A6.7, SERKAL, WNT-4 protein

Gen-ID: 54361, 22417

NCBI Accession: NP_110388

Pathways: WNT Signalweg, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cell-Cell Junction Organization, Tube Formation