SH3GLB1 Antikörper (C-Term)

Produktnummer ABIN184621

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch SH3GLB1 in WB, ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für SH3GLB1 Antikörper (C-Term) (ABIN184621)

Target: SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SH3GLB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-SH3GLB1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: BIF-1 / SH3GLB1

Sequenz: QKGKVPITYL ELLN

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QKGKVPITYLELLN, from the C Terminus of the protein sequence according to NP_057093.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of cell lines HEK293 and NIH3T3, and approx. 38 kDa in lysates of cell lines A431, RAW264.7 and KNRK (calculated MW of 40.8 kDa according to Human NP_057093.1, Mouse NP_062337.1 and Rat NP_001011929.1).

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SH3GLB1

Target: SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))

Andere Bezeichnung: SH3GLB1

Hintergrund: SH3GLB1, Bif-1, SH3-domain GRB2-like endophilin B1, CGI-61, KIAA0491, endophilin B1, Bax-interacting factor 1, SH3-containing protein SH3GLB1, SH3-domain, GRB2-like, endophilin B1, SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1, dJ612B15.2

Gen-ID: 51100, 54673

NCBI Accession: NP_057093

Pathways: Autophagie